Coding

Part:BBa_K112140:Design

Designed by: Madhvi Venkatesh   Group: iGEM08_UC_Berkeley   (2008-10-30)


attR1 rev-CamR-Lox-AmpR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1075
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1075
    Illegal NotI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1035
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1075
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1075
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1934


Design Notes

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page


Source

Anderson lab double antibiotic assembly vectors

References